8SY1

RNA duplex bound with imidazolium bridged GA dinucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	99
Detector technology	CCD
Collection date	2022-10-15
Detector	MAR CCD 130 mm
Wavelength(s)	0.9785
Spacegroup name	P 3
Unit cell lengths	43.656, 43.656, 81.126
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.290 - 1.760
R-factor	0.22543
Rwork	0.224
R-free	0.25633
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8swg
RMSD bond length	0.022
RMSD bond angle	2.837
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.820
High resolution limit [Å]	1.760	1.760
Rmerge	0.099	0.577
Rmeas	0.104	0.638
Number of reflections	15744	1726
<I/σ(I)>	19.6	2.3
Completeness [%]	91.9	100
Redundancy	5.5	5.5
CC(1/2)	0.986	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	0.5 M ammonium sulfate, 0.1 M sodium citrate tribasic dihydrate, pH 5.6, 1.0 M lithium sulfate monohydrate