8SY1
RNA duplex bound with imidazolium bridged GA dinucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2022-10-15 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9785 |
Spacegroup name | P 3 |
Unit cell lengths | 43.656, 43.656, 81.126 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.290 - 1.760 |
R-factor | 0.22543 |
Rwork | 0.224 |
R-free | 0.25633 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8swg |
RMSD bond length | 0.022 |
RMSD bond angle | 2.837 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.099 | 0.577 |
Rmeas | 0.104 | 0.638 |
Number of reflections | 15744 | 1726 |
<I/σ(I)> | 19.6 | 2.3 |
Completeness [%] | 91.9 | 100 |
Redundancy | 5.5 | 5.5 |
CC(1/2) | 0.986 | 0.825 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 0.5 M ammonium sulfate, 0.1 M sodium citrate tribasic dihydrate, pH 5.6, 1.0 M lithium sulfate monohydrate |