8SXS

Crystal structure of a Nudix hydrolase effector from Magnaporthe oryzae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	59.188, 60.545, 62.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.970 - 1.570
R-factor	0.1577
Rwork	0.156
R-free	0.19580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.018
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.500	1.620
High resolution limit [Å]	1.570	1.570
Rmerge	0.047	0.213
Rmeas	0.051	0.232
Rpim	0.020	0.089
Number of reflections	32124	3049
<I/σ(I)>	22.35	5.9
Completeness [%]	99.6	96.5
Redundancy	6.6	6.6
CC(1/2)	0.999	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	200 mM Potassium thiocyanate 20% w/v PEG 3350