8SXQ
Crystal structure of Sel-1 repeat protein LceB from Legionella pneumophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-02 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.18082 |
Spacegroup name | P 32 |
Unit cell lengths | 116.790, 116.790, 88.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.720 - 2.700 |
R-factor | 0.219 |
Rwork | 0.217 |
R-free | 0.25080 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond angle | 13.702 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.720 | 2.797 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.021 | |
Number of reflections | 36977 | 3696 |
<I/σ(I)> | 20.5 | |
Completeness [%] | 99.9 | |
Redundancy | 4.7 | |
CC(1/2) | 0.999 | 0.499 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293.15 | 7mg/mL 0.1M Citric Acid pH 4.0 10% MPD |