8SXP
Crystal structure of long neurotoxin from the venom of the king cobra (3FTx-L15) in complex with Fab of broadly neutralizing antibody 95Mat5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2023-03-23 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.860, 42.443, 103.356 |
Unit cell angles | 90.00, 103.70, 90.00 |
Refinement procedure
Resolution | 45.490 - 2.900 |
R-factor | 0.2638 |
Rwork | 0.261 |
R-free | 0.32000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.843 |
Data reduction software | HKL-2000 (v721.1) |
Data scaling software | HKL-2000 (v721.1) |
Phasing software | PHENIX (1.19.2-4158-000) |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.424 | |
Rpim | 0.354 | |
Number of reflections | 10741 | 98 |
<I/σ(I)> | 17.81 | |
Completeness [%] | 82.1 | |
Redundancy | 2.7 | |
CC(1/2) | 0.990 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.75 | 293.15 | 100mM sodium citrate pH 3.75, and 26% PEG6000 |