8SXC

Crystal Structure of Fluorescent Protein Fusion Red 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	71.155, 81.442, 42.539
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.310 - 1.500
R-factor	0.16253
Rwork	0.161
R-free	0.21927
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.937
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.085	0.074	0.907
Rmeas	0.092	0.080	1.011
Rpim	0.035	0.031	0.433
Total number of observations	267152
Number of reflections	39722	4218	3641
<I/σ(I)>	9.7
Completeness [%]	99.2	99.1	93
Redundancy	6.7	6.8	4.3
CC(1/2)	0.993	0.992	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% w/v PEG 6000, 1.0M LiCl, 0.1M HEPES, pH 7.0