8SX6
RNA duplex bound with GMP and AMP monomers
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2021-03-14 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.987 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 43.526, 43.526, 80.139 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.730 - 1.450 |
R-factor | 0.22118 |
Rwork | 0.220 |
R-free | 0.24181 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6c8n |
RMSD bond length | 0.028 |
RMSD bond angle | 3.611 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.122 | 0.531 |
Rmeas | 0.129 | 0.558 |
Rpim | 0.042 | 0.169 |
Number of reflections | 15931 | 1584 |
<I/σ(I)> | 121.5 | 7.9 |
Completeness [%] | 98.5 | 100 |
Redundancy | 10.7 | 10.8 |
CC(1/2) | 0.978 | 0.972 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 10% v/v MPD, 0.040 M sodium cacodylate trihydrate, pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.08 M sodium chloride, 0.012 M potassium chloride, 0.02 M magnesium chloride hexahydrate |