8SX6

RNA duplex bound with GMP and AMP monomers

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	99
Detector technology	CCD
Collection date	2021-03-14
Detector	MAR CCD 130 mm
Wavelength(s)	0.987
Spacegroup name	P 3 2 1
Unit cell lengths	43.526, 43.526, 80.139
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.730 - 1.450
R-factor	0.22118
Rwork	0.220
R-free	0.24181
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c8n
RMSD bond length	0.028
RMSD bond angle	3.611
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.450	1.450
Rmerge	0.122	0.531
Rmeas	0.129	0.558
Rpim	0.042	0.169
Number of reflections	15931	1584
<I/σ(I)>	121.5	7.9
Completeness [%]	98.5	100
Redundancy	10.7	10.8
CC(1/2)	0.978	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	10% v/v MPD, 0.040 M sodium cacodylate trihydrate, pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.08 M sodium chloride, 0.012 M potassium chloride, 0.02 M magnesium chloride hexahydrate