8SX5
GpppA dinucleotide binding to RNA CU template
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2022-10-15 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 44.522, 44.522, 85.524 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.520 - 1.950 |
| R-factor | 0.28576 |
| Rwork | 0.284 |
| R-free | 0.32316 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c8k |
| RMSD bond length | 0.019 |
| RMSD bond angle | 3.688 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.138 | 0.413 |
| Rmeas | 0.148 | 0.438 |
| Rpim | 0.052 | 0.143 |
| Number of reflections | 6922 | 756 |
| <I/σ(I)> | 10 | 13.5 |
| Completeness [%] | 100.0 | 90.8 |
| Redundancy | 8.9 | 9.4 |
| CC(1/2) | 0.987 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.05 M magnesium sulfate hydrate, 0.05 M HEPES sodium, pH 7.0, 1.6 M lithium sulfate monohydrate |
