8SX0
Bordetella filamentous hemagglutinin (FhaB) C-terminal domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795, 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.060, 46.576, 75.987 |
| Unit cell angles | 90.00, 91.69, 90.00 |
Refinement procedure
| Resolution | 39.710 - 1.650 |
| R-factor | 0.1815 |
| Rwork | 0.179 |
| R-free | 0.20430 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.580 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.060 | 0.805 |
| Rmeas | 0.071 | 0.952 |
| Rpim | 0.038 | 0.501 |
| Number of reflections | 19264 | 962 |
| <I/σ(I)> | 15 | 2.2 |
| Completeness [%] | 98.8 | 99.6 |
| Redundancy | 6.7 | 6.9 |
| CC(1/2) | 0.999 | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M phosphate citrate pH 5, 26.5% PEG 8000, 0.2 M NaCl |
