8SX0

Bordetella filamentous hemagglutinin (FhaB) C-terminal domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795, 1.0
Spacegroup name	P 1 21 1
Unit cell lengths	23.060, 46.576, 75.987
Unit cell angles	90.00, 91.69, 90.00

Refinement procedure

Resolution	39.710 - 1.650
R-factor	0.1815
Rwork	0.179
R-free	0.20430
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.580	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.060	0.805
Rmeas	0.071	0.952
Rpim	0.038	0.501
Number of reflections	19264	962
<I/σ(I)>	15	2.2
Completeness [%]	98.8	99.6
Redundancy	6.7	6.9
CC(1/2)	0.999	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M phosphate citrate pH 5, 26.5% PEG 8000, 0.2 M NaCl