8SWP
Structure of K. lactis PNP bound to hypoxanthine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.211, 110.825, 97.718 |
| Unit cell angles | 90.00, 108.06, 90.00 |
Refinement procedure
| Resolution | 39.610 - 2.100 |
| R-factor | 0.2013 |
| Rwork | 0.199 |
| R-free | 0.23590 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.546 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.082 | 0.029 | 0.913 |
| Total number of observations | 757592 | ||
| Number of reflections | 205816 | 9511 | 10389 |
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 98.9 | 91.5 | 99.1 |
| Redundancy | 3.7 | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 25% (w/v) PEG 3,350, 0.185 M Ammonium acetate and 0.1 M BIS-TRIS |
