8SWO
GpppA dinucleotide ligand binding to RNA UC template
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2022-10-15 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.987 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 44.121, 44.121, 84.530 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.190 - 1.640 |
R-factor | 0.24945 |
Rwork | 0.248 |
R-free | 0.27450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hbx |
RMSD bond length | 0.023 |
RMSD bond angle | 3.845 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.136 | 0.796 |
Number of reflections | 10994 | 1205 |
<I/σ(I)> | 10.8 | 8.1 |
Completeness [%] | 90.1 | 100 |
Redundancy | 10.1 | 10.4 |
CC(1/2) | 0.976 | 0.985 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.02 M magnesium chloride hexahydrate, 0.05 M MOPS, pH 7.0, 55% v/v Tacsimate, pH 7.0, 2 mM hexammine cobalt(III) chloride |