8SW1
Puromycin-sensitive aminopeptidase with bound peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.745, 256.562, 60.279 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.520 - 3.650 |
R-factor | 0.2603 |
Rwork | 0.257 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.438 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.840 |
High resolution limit [Å] | 3.650 | 6.980 | 3.650 |
Rmerge | 0.133 | 0.071 | 0.567 |
Rmeas | 0.156 | 0.083 | 0.669 |
Rpim | 0.079 | 0.042 | 0.346 |
Number of reflections | 13423 | 2059 | 1876 |
<I/σ(I)> | 6.3 | ||
Completeness [%] | 99.2 | 98 | 99.7 |
Redundancy | 3.6 | 3.4 | 3.6 |
CC(1/2) | 0.993 | 0.697 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 5-8 mg/ml PSA mixed 1:1 with 15% PEG 4K, 0.1 M Tris (pH 8.5), 0.5 M sodium chloride, and 1.5% v/v dioxane |