8SW0
Puromycin sensitive aminopeptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97182 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.684, 257.715, 60.387 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.201 - 2.301 |
| R-factor | 0.224 |
| Rwork | 0.223 |
| R-free | 0.24060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z1w |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.416 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.20) |
| Refinement software | PHENIX (1.20) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.065 | 0.035 | 0.587 |
| Rmeas | 0.073 | 0.039 | 0.664 |
| Rpim | 0.033 | 0.017 | 0.306 |
| Total number of observations | 223462 | ||
| Number of reflections | 47993 | 5334 | 4680 |
| <I/σ(I)> | 12.8 | ||
| Completeness [%] | 95.9 | 99.8 | 95.4 |
| Redundancy | 4.7 | 5 | 4.6 |
| CC(1/2) | 0.997 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 5-8 mg/ml PSA mixed 1:1 with 15% PEG 4K, 0.1 M Tris (pH 8.5), 0.5 M sodium chloride, and 1.5% v/v dioxane |
