8SVH

Ubiquitin variant i53 mutant L67R bound to 53BP1 Tudor Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95372
Spacegroup name	P 21 21 21
Unit cell lengths	40.460, 46.960, 90.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.340 - 1.160
Rwork	0.198
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.340	1.180
High resolution limit [Å]	1.160	1.160
Rmeas	0.093
Rpim	0.035
Number of reflections	60078	5857
<I/σ(I)>	12	3.35
Completeness [%]	99.8
Redundancy	7
CC(1/2)	0.995	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	0.1 M MES pH6.6, 0.2 M Trimethylamine N-oxide dehydrate, 7% w/v PEG2000