8SVH
Ubiquitin variant i53 mutant L67R bound to 53BP1 Tudor Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-12-08 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.95372 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.460, 46.960, 90.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.340 - 1.160 |
Rwork | 0.198 |
R-free | 0.21940 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.340 | 1.180 |
High resolution limit [Å] | 1.160 | 1.160 |
Rmeas | 0.093 | |
Rpim | 0.035 | |
Number of reflections | 60078 | 5857 |
<I/σ(I)> | 12 | 3.35 |
Completeness [%] | 99.8 | |
Redundancy | 7 | |
CC(1/2) | 0.995 | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 0.1 M MES pH6.6, 0.2 M Trimethylamine N-oxide dehydrate, 7% w/v PEG2000 |