8SVD

Structure of M. baixiangningiae DarR-DNA complex reveals novel dimer-of-dimers DNA binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	68.034, 155.758, 117.844
Unit cell angles	90.00, 97.26, 90.00

Refinement procedure

Resolution	47.450 - 3.490
R-factor	0.268
Rwork	0.267
R-free	0.28440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.823
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.450	3.580
High resolution limit [Å]	3.490	3.490
Rpim	0.061	0.253
Number of reflections	30500	2100
<I/σ(I)>	5.2	1.9
Completeness [%]	98.4
Redundancy	3.4
CC(1/2)	0.995	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium citrate tribasic dihydrate pH 5.0, 0.2 M magnesium chloride and 13% PEG 20,000