8SVC

Crystal Structure of SBP from Klebsiella pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920099
Spacegroup name	C 2 2 21
Unit cell lengths	62.187, 141.291, 71.388
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.918 - 1.590
R-factor	0.1853
Rwork	0.184
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.772
Data reduction software	autoPROC (1.0.5 (20211020))
Data scaling software	autoPROC
Phasing software	PHENIX (1.13_2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.920	1.647
High resolution limit [Å]	1.590	1.590
Rmerge	0.053
Rpim	0.053
Number of reflections	42016	4014
<I/σ(I)>	7.78
Completeness [%]	98.5
Redundancy	2
CC(1/2)	0.995	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Ammonium Sulfate and 23% (w/v) PEG 4000