8SV6

Structure of the M. smegmatis DarR protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	94.422, 94.422, 215.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.210 - 3.560
R-factor	0.2783
Rwork	0.275
R-free	0.30540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.618
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.860	3.600
High resolution limit [Å]	3.560	3.560
Rpim	0.053	0.561
Number of reflections	12397	1239
<I/σ(I)>	7	1.5
Completeness [%]	99.9
Redundancy	8
CC(1/2)	0.997	0.357

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	22% PEG 3350 and 0.1 M Citrate