8SUU
Crystal structure of YisK from Bacillus subtilis in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2019-11-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.937, 93.768, 125.484 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.930 - 2.260 |
| R-factor | 0.21376 |
| Rwork | 0.211 |
| R-free | 0.25478 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.141 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (2000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.930 | 2.320 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.117 | 2.209 |
| Rpim | 0.070 | 1.423 |
| Number of reflections | 30609 | 2217 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.998 | 0.298 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Mg-acetate and 20% (w/v) PEG 3350 |
