8SUD

JC Polyomavirus LTA NLS bound to importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.780, 89.760, 99.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 2.100
R-factor	0.194
Rwork	0.193
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uaf
RMSD bond length	0.003
RMSD bond angle	0.566
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.920	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.110	1.290
Rmeas	0.110	1.340
Rpim	0.030	0.380
Number of reflections	42050	3409
<I/σ(I)>	14.8	1.9
Completeness [%]	99.9
Redundancy	13.1
CC(1/2)	1.000	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.85 M sodium citrate, 0.1 M HEPES pH 7.5, 0.01 M DTT