8SUA
Structure of M. baixiangningiae DarR-ligand complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 85.437, 85.437, 62.436 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.720 - 1.600 |
R-factor | 0.1853 |
Rwork | 0.184 |
R-free | 0.20900 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.720 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Rpim | 0.020 | 0.406 |
Number of reflections | 35029 | 2647 |
<I/σ(I)> | 25.2 | 2.1 |
Completeness [%] | 99.9 | |
Redundancy | 11.2 | |
CC(1/2) | 1.000 | 0.805 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 273 | 1.5 M NaCl, 0.2 M MgCl2, 0.1 M Tris |