8STX
Crystal structure of the F337A mutation of Trypanosoma cruzi glucokinase in the apo form (open conformation)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.307, 78.654, 77.079 |
Unit cell angles | 90.00, 100.75, 90.00 |
Refinement procedure
Resolution | 39.582 - 2.400 |
Rwork | 0.202 |
R-free | 0.24460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.286 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmeas | 0.081 | 0.430 |
Rpim | 0.045 | 0.250 |
Number of reflections | 30375 | 1361 |
<I/σ(I)> | 20.1 | 1.9 |
Completeness [%] | 96.1 | 85.7 |
Redundancy | 3 | 2.5 |
CC(1/2) | 0.982 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.05 M Succinic Acid, 15% PEG3350, pH 7.0 |