8SST

ZnFs 1-7 of CCCTC-binding factor (CTCF) K365T Mutant Complexed with 23mer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	160.561, 41.596, 135.215
Unit cell angles	90.00, 105.61, 90.00

Refinement procedure

Resolution	40.170 - 2.190
R-factor	0.2214
Rwork	0.220
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.465
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.170	2.270
High resolution limit [Å]	2.190	2.190
Rmerge	0.121	1.315
Rpim	0.053
Number of reflections	43468	3842
<I/σ(I)>	13.3	1
Completeness [%]	97.2	87.4
Redundancy	5.8	3.2
CC(1/2)	0.992	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M Ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3,350