8SSR

ZnFs 3-11 of CCCTC-binding factor (CTCF) Complexed with 35mer DNA 35-20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	362.211, 68.090, 61.988
Unit cell angles	90.00, 94.87, 90.00

Refinement procedure

Resolution	37.590 - 3.140
R-factor	0.2509
Rwork	0.249
R-free	0.28410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.481
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.590	3.260
High resolution limit [Å]	3.140	3.140
Rpim	0.061	0.338
Number of reflections	46861	3269
<I/σ(I)>	10.9	1.1
Completeness [%]	95.7	73.6
Redundancy	15.8	7.1
CC(1/2)	0.940	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	20-25%% w/v PEG 3,350, 0.1 M BIS-TRIS pH 5.0-6.5, 0.05-0.20M NaCl