8SSJ

Room-temperature X-ray structure of human mitochondrial serine hydroxymethyltransferase (hSHMT2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	293
Detector technology	PIXEL
Collection date	2023-02-24
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	161.609, 161.609, 210.784
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.650 - 2.500
R-factor	0.1737
Rwork	0.172
R-free	0.20460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.522
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2-4158-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rpim	0.063	0.291
Number of reflections	55062	5150
<I/σ(I)>	10.5
Completeness [%]	97.0
Redundancy	6
CC(1/2)	0.980	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	287.15	0.1 M Tricine pH 8.4, 15% PEG 3350