8SSG
Minimal protein-only/RNA-free Ribonuclease P from Hydrogenobacter thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03317 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 102.317, 113.764, 155.306 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.739 - 3.200 |
Rwork | 0.198 |
R-free | 0.24850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7f3e |
RMSD bond length | 0.009 |
RMSD bond angle | 2.076 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.840 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.116 | 1.799 |
Rmeas | 0.137 | 2.104 |
Rpim | 0.071 | 1.082 |
Number of reflections | 30570 | 4394 |
<I/σ(I)> | 12.1 | |
Completeness [%] | 99.9 | |
Redundancy | 6.8 | 7.2 |
CC(1/2) | 0.999 | 0.430 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277.15 | 1.0 M Sodium Malonate, pH 5.0, 0.1 M Sodium Acetate trihydrate, pH 4.5, 2% (w/v) polyethylene glycol 20,000 |