8SSF
Minimal protein-only/RNA-free Ribonuclease P from Hydrogenobacter thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03317 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.579, 109.214, 106.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.720 - 2.500 |
| Rwork | 0.200 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f3e |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.900 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.720 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.304 | 4.001 |
| Rmeas | 0.324 | 4.260 |
| Rpim | 0.111 | 1.443 |
| Total number of observations | 159064 | 18292 |
| Number of reflections | 18589 | 2076 |
| <I/σ(I)> | 6.3 | 1.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 8.6 | 8.8 |
| CC(1/2) | 0.991 | 0.434 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277.15 | 2 M ammonium sulfate, 0.1 M Bis-Tris, pH 5.5 |
