8SRZ

Structure of a bacterial death-like domain from Lysobacter enzymogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-05-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 65
Unit cell lengths	58.252, 58.252, 122.354
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.920 - 1.250
R-factor	0.1591
Rwork	0.159
R-free	0.17760
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.549
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.19.1_4122)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.640	1.270
High resolution limit [Å]	1.250	1.250
Rpim	0.014
Number of reflections	64141	64141
<I/σ(I)>	23
Completeness [%]	99.0
Redundancy	17.3
CC(1/2)	1.000	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	4 M sodium formate