8SRN
De novo designed transmembrane antiparallel homo-dimer G-X6-G 01322-42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-31 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 54.008, 71.408, 101.064 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.626 - 3.275 |
R-factor | 0.2531 |
Rwork | 0.253 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.757 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.000 | 60.000 | 3.360 |
High resolution limit [Å] | 3.275 | 8.950 | 3.275 |
Rmerge | 0.094 | 0.051 | 0.469 |
Rmeas | 0.099 | 0.054 | 0.498 |
Rpim | 0.030 | 0.018 | 0.158 |
Number of reflections | 3003 | 183 | 116 |
<I/σ(I)> | 7.2 | ||
Completeness [%] | 93.7 | 98.4 | 78.4 |
Redundancy | 9.6 | 9.4 | 7.4 |
CC(1/2) | 1.000 | 0.996 | 0.855 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Sodium chloride, 0.1 M Lithium sulfate, 0.1 M Sodium citrate pH 3.5, 30% PEG400 |