8SR6
Crystal structure of legAS4 from Legionella pneumophila subsp. pneumophila with histone H3 (3-17)peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-09-05 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.1806 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 69.320, 69.320, 196.010 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.210 - 2.220 |
R-factor | 0.1873 |
Rwork | 0.185 |
R-free | 0.22990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.596 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.210 | 2.340 |
High resolution limit [Å] | 2.220 | 2.220 |
Number of reflections | 27729 | 3836 |
<I/σ(I)> | 14.41 | |
Completeness [%] | 99.5 | |
Redundancy | 8.4 | |
CC(1/2) | 0.997 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M Bis-Tris 6.5 and 38% PPG P400 |