8SR1

particulate methane monooxygenase crosslinked with 4,4,4-trifluorobutanol bound

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	OTHER
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.18
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION