8SPA
Structural insights into cellular control of the human CPEB3 prion, functionally regulated by a labile-amyloid-forming segment
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3 |
| Software | RELION,EPU,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
