8SP9
Crystal Structure of Coxsackievirus B3 (CVB3) Cloverleaf RNA with tRNA scaffold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.429, 28.359, 113.111 |
| Unit cell angles | 90.00, 92.01, 90.00 |
Refinement procedure
| Resolution | 44.690 - 2.920 |
| R-factor | 0.2917 |
| Rwork | 0.291 |
| R-free | 0.30820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.688 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.920 |
| Rpim | 0.094 | 0.401 |
| Number of reflections | 9250 | 232 |
| <I/σ(I)> | 5.74 | |
| Completeness [%] | 83.3 | |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 100 mM Bis-Tris, pH 6.5, 48% polypropylene glycol (PPG)-P400 |
