8SOW
Structure of the complex formed by human interleukin-2 and scFv F10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.047, 40.215, 68.123 |
| Unit cell angles | 90.00, 111.21, 90.00 |
Refinement procedure
| Resolution | 38.450 - 1.710 |
| Rwork | 0.216 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.153 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.450 | 1.790 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.072 | 0.968 |
| Number of reflections | 37142 | 3657 |
| <I/σ(I)> | 12.68 | |
| Completeness [%] | 98.7 | 98.33 |
| Redundancy | 6.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 15-30% PEG 3350, 0.1-0.35 M ammonium fluoride |
