8SNZ
X-ray Crystal Structure of FMN-bound long-chain flavodoxin from Rhodopseudomonas palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-03 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.692, 58.878, 60.898 |
| Unit cell angles | 90.00, 105.47, 90.00 |
Refinement procedure
| Resolution | 29.350 - 2.170 |
| R-factor | 0.2303 |
| Rwork | 0.229 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.391 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.350 | 2.248 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 16973 | 1672 |
| <I/σ(I)> | 22.71 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.7 | |
| CC(1/2) | 0.983 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 0.3-0.6M magnesium formate, 15-20% polyethylene glycol 3350 |
