8SNP
Crystal structure of mouse Netrin-1 in complex with samarium ions
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2005-10-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0349 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.500, 69.500, 333.641 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.620 - 3.400 |
| R-factor | 0.2505 |
| Rwork | 0.250 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.873 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.700 | 3.670 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmeas | 0.255 | |
| Number of reflections | 13728 | 2782 |
| <I/σ(I)> | 5.8 | 1.9 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 8.3 | 8.5 |
| CC(1/2) | 0.994 | 0.923 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 293 | 0.1M HEPES pH 7.7, 2.8M NaCl, 0.2M glycine |
