8SMO
Crystal structure of the complex between truncated MLLE domain of PABPC1 and engineered superPAM2 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-27 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 1.000 |
Spacegroup name | P 31 |
Unit cell lengths | 108.275, 108.275, 75.866 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.110 - 3.000 |
R-factor | 0.2594 |
Rwork | 0.256 |
R-free | 0.29160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.520 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 27587 | 535 |
<I/σ(I)> | 17.1 | 0.9 |
Completeness [%] | 99.9 | |
Redundancy | 9.7 | 6.7 |
CC(1/2) | 0.995 | 0.385 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1 M citric acid pH 4.0, 1.6 M ammonium sulfate |