8SMF
Structure of SPO1 phage Tad2 in complex with 1''-3' gcADPR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2023-02-06 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.389, 82.472, 90.757 |
| Unit cell angles | 90.00, 107.88, 90.00 |
Refinement procedure
| Resolution | 46.060 - 1.750 |
| R-factor | 0.2088 |
| Rwork | 0.208 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8sme |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.793 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.060 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rpim | 0.057 | 0.630 |
| Number of reflections | 65939 | 3546 |
| <I/σ(I)> | 6.5 | 1.1 |
| Completeness [%] | 98.8 | 97.1 |
| Redundancy | 5.2 | 4.7 |
| CC(1/2) | 0.996 | 0.634 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | magnesium nitrate, PEG 3350 |
