8SM9
Air-oxidized C. fi TruffO expressed from M9 minimal medium supplemented with Fe
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.74 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 112.818, 67.880, 138.508 |
Unit cell angles | 90.00, 111.74, 90.00 |
Refinement procedure
Resolution | 60.040 - 2.300 |
R-factor | 0.2041 |
Rwork | 0.203 |
R-free | 0.25090 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.066 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.040 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 82002 | 7943 |
<I/σ(I)> | 3.28 | |
Completeness [%] | 94.0 | |
Redundancy | 6.1 | |
CC(1/2) | 0.958 | 0.497 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 0.07M Ammonium acetate, 5.6% PEG 4000, 20% glycerol |