8SM7

Air-oxidized G. y4 TruffO expressed from M9 minimal medium supplemented with Fe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.74
Spacegroup name	P 41 21 2
Unit cell lengths	127.369, 127.369, 217.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.780 - 2.200
R-factor	0.1997
Rwork	0.199
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.008
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.780	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	89804	7833
<I/σ(I)>	7.55
Completeness [%]	98.2
Redundancy	9.9
CC(1/2)	0.995	0.379

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	3.6 M sodium formate, 10% glycerol