8SM5

Crystal Structure of BHRF1 from Epstein Barr Virus in complex with BID BH3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979180
Spacegroup name	P 65
Unit cell lengths	186.817, 186.817, 70.486
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	80.894 - 2.610
R-factor	0.200666449516
Rwork	0.198
R-free	0.25966
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.193
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	161.790	2.710
High resolution limit [Å]	2.610	2.610
Number of reflections	42919	4267
<I/σ(I)>	19.6
Completeness [%]	99.9
Redundancy	11.3
CC(1/2)	0.998	0.611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1 mM NaC2H3O2 pH 5.0, 1.6 mM HCOONa