8SM3
Structure of Bacillus cereus VD045 Gabija GajA-GajB Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2022-10-19 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 215.791, 215.791, 173.807 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.240 - 3.000 |
R-factor | 0.2381 |
Rwork | 0.237 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.442 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.240 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.163 | 3.069 |
Number of reflections | 48144 | 4353 |
<I/σ(I)> | 15.4 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.1 | |
CC(1/2) | 0.999 | 0.624 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM HEPES-NaOH pH 7.5, 2.4% PEG-400, and 2.2 M ammonium sulfate |