8SM2
Crystal Structure of the macaque FcalphaRI bound to macaque IgA Fc.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 160.047, 160.047, 52.563 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.290 - 3.150 |
| R-factor | 0.2737 |
| Rwork | 0.273 |
| R-free | 0.29580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.261 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.104 | 0.819 |
| Rpim | 0.046 | 0.542 |
| Number of reflections | 12135 | 481 |
| <I/σ(I)> | 25.7 | 0.7 |
| Completeness [%] | 98.0 | 81.9 |
| Redundancy | 5.8 | 3.9 |
| CC(1/2) | 0.990 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 100 mM ammonium sulfate 12% PEG 3350 100 mM Tris-HCl pH 8.5 |
