8SLR

Crystal Structure of mouse TRAIL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	173
Detector technology	PIXEL
Collection date	2022-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 41 3 2
Unit cell lengths	147.350, 147.350, 147.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.430 - 2.400
R-factor	0.1609
Rwork	0.160
R-free	0.18250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1du3
RMSD bond length	0.006
RMSD bond angle	0.816
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	22033	1097
<I/σ(I)>	4.6	1.8
Completeness [%]	99.9	100
Redundancy	9.4	9.9
CC(1/2)	0.994	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	Protein: 4.4mg/ml Trail+ 1mM 12merNS2S6S in 25mM HEPES pH 7.1, 150mM NaCl Reservoir: 0.085M HEPES pH 7.5, 8.5% PEG 8000, 8.5%EG