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8SLB

X-ray structure of CorA N-terminal domain in complex with conformation-specific synthetic antibody C12

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 23-ID-B
Synchrotron siteAPS
Beamline23-ID-B
Temperature [K]100
Detector technologyPIXEL
Collection date2015-06-15
DetectorDECTRIS EIGER2 X 16M
Wavelength(s)1.0332
Spacegroup nameI 1 2 1
Unit cell lengths137.338, 44.527, 150.634
Unit cell angles90.00, 108.01, 90.00
Refinement procedure
Resolution45.820 - 2.040
R-factor0.16826
Rwork0.166
R-free0.21785
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.014
RMSD bond angle1.817
Data reduction softwareXDS (20190417)
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0258)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]45.8202.090
High resolution limit [Å]2.0382.040
Rmerge0.0560.657
Rmeas0.0780.918
Rpim0.0540.637
Number of reflections556784041
<I/σ(I)>13.1
Completeness [%]99.4
Redundancy3.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8.82938% dextrane sulfate, 17% PEG 3350, 0.02 M citric acid/0.08 M bis-Tris propane pH 8.8

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