8SL5
[2T13] Self-assembling left-handed two-turn tensegrity triangle with 13 interjunction base pairs and R3 symmetry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-19 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.99187 |
Spacegroup name | H 3 |
Unit cell lengths | 111.886, 111.886, 69.191 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.300 - 6.550 |
R-factor | 0.2055 |
Rwork | 0.203 |
R-free | 0.34880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8sjn |
RMSD bond length | 0.005 |
RMSD bond angle | 0.687 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.943 | 55.943 | 8.851 |
High resolution limit [Å] | 6.548 | 8.897 | 6.548 |
Number of reflections | 496 | 248 | 248 |
<I/σ(I)> | 5.1 | 8.8 | 1.4 |
Completeness [%] | 81.7 | ||
Redundancy | 9.7 | ||
CC(1/2) | 0.990 | 0.989 | 0.844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 100 mM MOPS, 1.25 M magnesium sulfate |