8SL5

[2T13] Self-assembling left-handed two-turn tensegrity triangle with 13 interjunction base pairs and R3 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.99187
Spacegroup name	H 3
Unit cell lengths	111.886, 111.886, 69.191
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.300 - 6.550
R-factor	0.2055
Rwork	0.203
R-free	0.34880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8sjn
RMSD bond length	0.005
RMSD bond angle	0.687
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.943	55.943	8.851
High resolution limit [Å]	6.548	8.897	6.548
Number of reflections	496	248	248
<I/σ(I)>	5.1	8.8	1.4
Completeness [%]	81.7
Redundancy	9.7
CC(1/2)	0.990	0.989	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	100 mM MOPS, 1.25 M magnesium sulfate