8SKJ
Crystal structure of a Nanobody bound to the V5 peptide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2022-09-22 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 |
Unit cell lengths | 45.430, 45.430, 70.600 |
Unit cell angles | 90.00, 90.01, 97.94 |
Refinement procedure
Resolution | 22.780 - 2.010 |
R-factor | 0.1917 |
Rwork | 0.189 |
R-free | 0.23620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.089 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.590 | 2.080 |
High resolution limit [Å] | 2.010 | 2.010 |
Number of reflections | 35510 | 1253 |
<I/σ(I)> | 24.2 | |
Completeness [%] | 95.1 | |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | Bis-Tris pH 6.5, 19% (w/v) PEG 3350 and 20% (v/v) ethylene glycol |