8SKJ
Crystal structure of a Nanobody bound to the V5 peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-09-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.430, 45.430, 70.600 |
| Unit cell angles | 90.00, 90.01, 97.94 |
Refinement procedure
| Resolution | 22.780 - 2.010 |
| R-factor | 0.1917 |
| Rwork | 0.189 |
| R-free | 0.23620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.089 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.590 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 35510 | 1253 |
| <I/σ(I)> | 24.2 | |
| Completeness [%] | 95.1 | |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | Bis-Tris pH 6.5, 19% (w/v) PEG 3350 and 20% (v/v) ethylene glycol |
