8SJT

[3T14+10] Self-assembling left-handed tensegrity triangle with 14 interjunction base pairs and a 10 bp linker with R3 symmetry

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-08
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00718
Spacegroup name	H 3
Unit cell lengths	212.243, 212.243, 164.272
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.660 - 9.380
R-factor	0.1795
Rwork	0.178
R-free	0.20290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w6w
RMSD bond length	0.008
RMSD bond angle	1.706
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.205	80.205	10.571
High resolution limit [Å]	9.380	19.137	9.380
Number of reflections	1416	203	201
<I/σ(I)>	7.3	19.7
Completeness [%]	85.5	100
Redundancy	10.1	8.8	10.2
CC(1/2)	0.999	0.999	0.038

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	100 mM MOPS, 1.25 M magnesium sulfate