8SJD
Cryo-EM structure of the Hermes transposase bound to two right-ends of its DNA transposon.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.1 |
| Number of Particle | 2920000 |
| Software | crYOLO,PHENIX,RELION,SerialEM,Gctf,UCSF Chimera,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
