8SJ7

Crystal structure of FBF-2 (RBD+CT) in complex with compact FBE RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	93.444, 93.444, 110.517
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.030 - 2.090
R-factor	0.1673
Rwork	0.165
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.653
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.130
High resolution limit [Å]	2.090	2.090
Rmeas	0.146
Rpim	0.050	0.577
Number of reflections	32508	1636
<I/σ(I)>	18	1.7
Completeness [%]	99.9
Redundancy	8.7
CC(1/2)	0.998	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30% PEG400, 0.1 M Tris, pH 8.5