8SJ6
Ara h 2.01 38B7 8F3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-03-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 44.400, 182.892, 194.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.090 - 3.970 |
| R-factor | 0.2538 |
| Rwork | 0.252 |
| R-free | 0.30180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.445 |
| Data reduction software | XDS (VERSION Feb 5, 2021 BUILT=20210205) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.100 | 4.110 |
| High resolution limit [Å] | 3.970 | 3.970 |
| Rmerge | 0.123 | 0.652 |
| Number of reflections | 57338 | 5774 |
| <I/σ(I)> | 10.2 | 2.3 |
| Completeness [%] | 97.5 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.996 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 21% p8K, 200 mM NaCl, 50 mM NaPi, 50 mM Citrate, pH 4.2 |
