8SIU

Origin Recognition Complex Associated (ORCA) protein bound to Orc2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-02
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.979092
Spacegroup name	C 2 2 21
Unit cell lengths	91.023, 135.431, 78.567
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.510 - 1.800
R-factor	0.1712
Rwork	0.170
R-free	0.20320
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.091
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.510	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	45458	2577
<I/σ(I)>	14.8	1.1
Completeness [%]	99.8	97.4
Redundancy	39
CC(1/2)	0.999	0.505

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	1.2 M ammonium sulfate, 100 mM MES pH 5.3